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AssigneesUniversity of Georgia Research Foundation, Inc., North Carolina State University
InventorsMichael W. W. Adams, Robert M. Kelly, Aaron B. Hawkins, Angeli Lal Menon, Gina Lynette Pries Lipscomb, Gerrit Jan Schut
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Provided herein are genetically engineered microbes that include at least a portion of a carbon fixation pathway, and in one embodiment, use molecular hydrogen to drive carbon dioxide fixation. In one embodiment, the genetically engineered microbe is modified to convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof at levels greater than a control microbe. Other products may also be produced. Also provided herein are cell free compositions that convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof. Also provided herein are methods of using the genetically engineered microbes and the cell free compositions.
A genetically engineered microbe modified to convert acetyl CoA, molecular hydrogen and carbon dioxide to 4-hydroxybutyrate, wherein the 4-hydroxybutyrate is produced at increased levels compared to a control microbe, wherein the microbe is a hyperthermophile, wherein the microbe is a member of the domain Archaea or a member of the domain Bacteria, and wherein the microbe comprises an exogenous polynucleotide encoding a polypeptide, wherein the polypeptide has an activity selected from 3-hydroxypropionate:CoA ligase activity, 3-hydroxypropionyl-CoA dehydratase activity, acryloyl-CoA reductase activity, methylmalonyl-CoA epimerase activity, methylmalonyl-CoA mutase activity, and succinate semialdehyde reductase activity.
Dependencies are inferred from the claim text.