Plant synthesizing hypoallergenic paucimannose type N-glycan and uses thereof

A method for producing a transgenic Arabidopsis plant for production of a protein containing a paucimannose-type N-glycan that does not include α1,3-fucose and β1,2-xylose residues but includes an α1,6-fucose residue, the method comprising:

• preparing a quadruple mutant (qm) Arabidopsis plant having mutations that knock_out each of four endogenous genes to produce a protein containing a paucimannose-type N-glycan that does not include α1,3-fucose and β1,2-xylose residues;
• and
• transforming the quadruple mutant Arabidopsis plant with a recombinant vector containing a transgene encoding the α1,6-fucosyltransferase (FUT8) consisting of SEQ ID NO:
• 10 to overexpress the FUT8 gene
• wherein the four endogenous genes are:
• the α1,3-fucosyltransferase A (FucTA) gene encoding an α1,3-fucosyltransferase A (FucTA) consisting of SEQ ID NO:
• 2;
• the α1,3-fucosyltransferase B (FucTB) gene encoding an α1,3-fucosyltransferase B (FucTB) consisting of SEQ ID NO:
• 4;
• the β1,2-xylosyltransferase (XylT) gene encoding a β1,2-xylosyltransferase (XylT) consisting of SEQ ID NO:
• 6;
• and
• the β1,2-N-acetylglucosaminyltransferase II (GnTII) gene encoding a β1,2-N-acetylglucosaminyltransferase II (GnTII) consisting of SEQ ID NO:
• 8.