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US 10,214,752 B2Granted
Biosynthesis of 1,3-butanediol
AssigneeInvista North America S.A.R.L.
InventorPawel Mordaka
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Abstract
This document describes biochemical pathways for producing 1,3-butanediol using a polypetide having β-ketothiolase activity to form a 3-oxo-5-hydroxypentanoyl-CoA intermediate that can be enzymatically converted to 1,3-butanediol, as well as recombinant hosts producing 1,3-butanediol.
Claims2
A method of producing 3-oxo-5-hydroxypentanoyl-CoA in a recombinant host, said method comprising:
- enzymatically producing 3-hydroxypropionyl-CoA from malonyl-CoA using a polypeptide having CoA transferase activity, wherein said polypeptide having a CoA transferase has at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 5, 6, 7, 12 or 13;
- and
- enzymatically converting 3-hydroxypropionyl-CoA to 3-oxo-5-hydroxypentanoyl-CoA using a polypeptide having ß-ketothiolase activity classified under EC. 2.3.1.-, wherein said polypeptide having ß-ketothiolase activity has at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NOs:
- 1 or 2 and is capable of converting 3-hydroxypropionyl-CoA to 3-oxo-5-hydroxypentanoyl-CoA,
- wherein at least one of said polypeptides is encoded by an exogenous nucleic acid sequence.
3 more elements
Dependencies are inferred from the claim text.