Methods and compositions for inhibiting retinopathy of prematurity
AssigneeUniversity of Utah Research Foundation
InventorMary Elizabeth Hartnett
Life of this patent
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Abstract
Disclosed are vectors and compositions comprising a pol II promoter and an shRNA wherein the shRNA has a sense RNA strand and an antisense RNA strand, wherein the sense and the antisense RNA strands form an RNA duplex, and wherein the sense RNA strand comprises a nucleotide sequence identical to a target sequence in STAT3, VEGFR, or EPOR. Also disclosed are methods of treating retinopathy of prematurity (ROP), methods of inhibiting expression of STAT3, VEGFR, and EPOR, and methods of regulating signaling events associated with intravitreal neovascularization (IVNV).
Claims10
A method of treating retinopathy of prematurity (ROP) comprising administering to a subject a composition comprising a vector, wherein the vector comprises a polymerase II (pol II) promoter and a first shRNA, wherein the first shRNA is embedded in microRNA, and wherein the first shRNA has a sense RNA strand and an antisense RNA strand, wherein the sense and the antisense RNA strands form an RNA duplex, wherein the sense RNA strand comprises a nucleotide sequence identical to a target sequence in STAT3, VEGFR2, or EPOR mRNA, and wherein the composition is administered via subretinal injection, wherein the first shRNA consists of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:25, SEQ ID NO:26, or SEQ ID NO:27.
Dependencies are inferred from the claim text.