Method for treatment and control of plant disease
AssigneeThe Texas A&M University System
InventorsCarlos F. Gonzalez, Stephen J. Ahern, Mayukh Das, Ryland F. Young, III, Tushar Suvra Bhowmick
Life of this patent
Estimating the term…
Abstract
The present invention provides methods for development of a virulent bacteriophage-based treatment for the control of plant diseases caused by Xylella fastidiosa. The invention further provides methods of isolating and propagating bacteriophage virulent to X. fastidiosa in a Xanthomonas bacterial host and for treating or reducing symptoms of X. fastidiosa infection in a plant. The invention further provides methods of isolating and propagating bacteriophage virulent to Xanthomonas axonopodis pv. citri and for treating or reducing symptoms of Xanthomonas axonopodis pv. citri infection in a plant.
Claims13
A method of preventing or reducing symptoms or disease associated with Xanthomonas axonopodis pv. citri or Xylella fastidiosa in a plant, comprising contacting said plant with a population of virulent bacteriophage particles that includes Xylella fastidiosa in its host range, wherein the virulent bacteriophage is selected from the group consisting of:
- the Xfas100 phage type and the Xfas300 phage type;
- wherein the Xfas100 type phage has the following characteristics:
- (a) the bacteriophage is capable of lysing said Xylella fastidiosa and/or Xanthomonas bacteria;
- (b) the bacteriophage infects a cell by binding to a Type IV pilus;
- (c) the phage belongs to Siphoviridae family and has capsid size ranging from 55-77 nm in diameter;
- (d) the genomic size of bacteriophage is about 55500 bp to 56200 bp;
- and
- (e) the bacteriophage prevents or reduces symptoms associated with X. fastidiosa or Xanthomonas axonopodis pv. citri and subspecies thereof, in a plant or plants;
- and wherein the Xfas300 type phage has the following characteristics:
- (f) the bacteriophage is capable of lysing said Xylella fastidiosa and/or Xanthomonas bacteria;
- (g) the bacteriophage infects a cell by binding to a Type IV pilus;
- (h) the phage belongs to Podoviridae family and has capsid size ranging from 58-68 nm in diameter;
- (i) the genomic size of bacteriophage is about 43300 bp to 44600 bp;
- and
- (j) the bacteriophage prevents or reduces symptoms associated with X. fastidiosa or Xanthomonas axonopodis pv. citri and subspecies thereof, in a plant or plants.
Dependencies are inferred from the claim text.