Lipoprotein nanoplatelets: fluorescent, zwitterionic probes for molecular and cellular imaging
AssigneeThe Board of Trustees of the University of Illinois
InventorsAndrew Smith, Sung Jun Lim, Aditi Das
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Abstract
Compositions are provided comprising water-stable semi-conductor nanoplatelets encapsulated in a hydrophilic coating further comprising lipids and lipoproteins. Uses include biomolecular imaging and sensing, and methods of making comprise: colloidal synthesis of CdSe core NPLs; layer-by-layer growth of a CdS shell; and encapsulation of CdSe/CdScore/shell NPLs in lipid and lipoprotein components through an evaporation-encapsulation using zwitterionic phospholipids, detergents, and amphipathic membrane scaffold proteins.
Claims13
A method for making a lipoprotein nanoplatelet (NPL) composition comprising:
- colloidal synthesis of NPL semiconductor cores;
- layer-by-layer growth of NPL semiconductor shells;
- and
- encapsulation of core/shell NPLs in lipid hydrophilic coating components through an evaporation-encapsulation using zwitterionic phospholipids, detergents, and amphipathic lipoproteins,
- wherein the hydrophilic coating components comprise phospholipids and lipoproteins,
- wherein the phospholipid is selected from 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and the lipoprotein is the membrane scaffold protein MSP1E3D1,
- wherein the phospholipids DMPC or (POPC) are dispersed in CHCl3 and added in a 10,000:1 phospholipid:nanoplatelet molar ratio,
- and wherein the MSP1E3D1 is added in a 100:1 MSP1E3D1:nanoplatelet molar ratio.
Dependencies are inferred from the claim text.