Chips, detection systems, and methods for multiplex pneumococcus serology

An arrayed imaging reflectometry (AIR) sensor chip suitable for serological detection of Streptococcus pneumoniae comprising:

• a multilayer substrate comprising a surface;
• and
• antibodies or antibody binding fragments covalently bound to said surface at different locations, wherein each antibody or antibody binding fragment binds specifically to one of two or more different serotypes of Streptococcus pneumoniae, wherein the surface forms a coating that results in destructive interference of polarized light illuminating the surface at an appropriate incident angle and wavelength in the absence of Streptococcus pneumoniae binding, and wherein exposure of the surface to a sample comprising Streptococcus pneumoniae to which the antibodies or antibody binding fragments bind produces a detectable change in reflectance at a location upon antibody or antibody binding fragment binding.