Methods, reagents and kits for flow cytometric immunophenotyping

A reagent composition for flow cytometric immunophenotyping of leukocytes and characterization of normal and aberrant subpopulations thereof, wherein the reagent composition comprises fluorochrome-conjugated antibodies directed against the following combination of markers:

• CD20, CD4, CD45, CD19, Igλ, CD8, Igκ, CD56, CD5, T-cell receptor (TCR) γδ, CD3, and CD38, wherein:
• (i) the antibody against CD20 and the antibody against CD4 are both conjugated to the same fluorochrome;
• (ii) the antibody against Igλ and the antibody against CD8 are both conjugated to the same fluorochrome;
• (iii) the antibody against CD19 and the antibody against TCRγδ are both conjugated to the same fluorochrome, and
• (iv) the antibody against Igκ and the antibody against CD56 are both conjugated to the same fluorochrome, and
• wherein:
• the antibody against CD45, the antibody against CD5, the antibody against CD3, and the antibody against CD38 are each conjugated to a different fluorochrome;
• and
• the fluorochrome in (i), the fluorochrome in (ii), the fluorochrome in (iii), the fluorochrome in (iv), the fluorochrome attached to the antibody against CD45, the fluorochrome attached to the antibody against CD5, the fluorochrome attached to the antibody against CD3, and the fluorochrome attached to the antibody against CD38, each having a distinguishably distinct fluorescence emission.