Mixer bypass sample injection for liquid chromatography
AssigneeAgilent Technologies, Inc.
InventorRobert William Albrecht, Jr.
Life of this patent
Estimating the term…
Abstract
In a liquid chromatography system, a sample is injected into a column by flowing a solvent mixture from a mixer into the column along a solvent mixture flow path; and injecting a sample into the solvent mixture flow path downstream of the mixer. In another liquid chromatography system, a sample is injected into a column by loading an isolator fluid into a sample loop, loading a sample into the sample loop, and flowing the sample into the column as a plug in front of the isolator fluid.
Claims20
A method for injecting a sample into a flow of a liquid chromatography system, the method comprising:
- providing a flow of a mobile phase in a mobile phase flow path at a mobile phase pressure;
- providing a fluidic junction in the mobile phase flow path, the fluidic junction communicating with a sample injection line and a chromatographic column;
- pressurizing a sample injection flow path, containing a sample, to a pressure matching the mobile phase pressure;
- connecting the sample injection flow path to the mobile phase flow path via the sample injection line and the fluidic junction;
- combining a flow of the pressurized sample injection flow path with the flow of the mobile phase at the fluidic junction to control a degree of sample-band focusing.
A method for injecting a sample into a solvent mixture path to a separation column of a liquid chromatography system, the method comprising:
- establishing a solvent mixture flow into the column along a solvent mixture flow path at a solvent mixture flow path pressure;
- providing a fluidic junction in the mobile phase flow path, the fluidic junction communicating with a sample injection line and a chromatographic column;
- pressurizing a sample injection flow path, containing a sample, to a pressure matching the solvent mixture flow path pressure;
- connecting the pressurized sample injection flow path to the solvent mixture flow path via the sample injection line and the fluidic junction;
- and
- controlling a degree of sample-band focusing by controlling a pumping of the sample in the pressurized sample injection flow path into the solvent mixture flow path at the fluidic junction.
A system for injecting a sample into a flow of a liquid chromatography system, the system comprising:
- a chromatographic column;
- a syringe configured to dispense a sample;
- a first fluid channel configured to conduct a mobile phase;
- a second fluid channel configured to receive the sample, and communicating with the syringe;
- a junction comprising a first port communicating with the first fluid channel, a second port communicating with the second fluid channel, and a third port communicating with the chromatographic column;
- and
- a controller configured to control pressurizing the second fluid channel and to control a degree of sample-band focusing by controlling a combining of the flow of the pressurized second fluid channel with a flow of the mobile phase in the first fluid channel, wherein the sample flows with the mobile phase into the chromatographic column.
Dependencies are inferred from the claim text.