Optical system for parameter characterization of an element of body fluid or tissue

An optical system for parameter characterization of an element of body fluid or tissue comprising an optical device which comprises:

• a light source for emitting light onto the element;
• a chamber for holding an element of body fluid or tissue, wherein the chamber includes a reference sample;
• a sample spectrometer for recording the spectrum of light from the element of body fluid or tissue, said light from the element being of transmittance, reflectance or Raman scattering of the emitted light by said element and the spectrum of light from the reference sample;
• the optical system further comprising a processor having a memory and configured
• to compare the recorded spectrum of the reference sample to a stored value within the memory, by obtaining a higher spectral full width at half maximum (FWHM) than that of an optical limit of the spectrometer by:
• (1) calculating an interpolation model of the higher pixel resolution by local regression, and interpolating the obtained reference spectral measurements;
• (2) performing deconvolution of the interpolated spectral measurements by an apodization function of the optical device to resolve convolved information below optical resolution;
• and
• (3) comparing the convoluted spectral measurements to a stored reference spectral measurement of the reference sample,
• where the convoluted spectral measurement of the reference sample is substantially similar to the stored reference measurement, convert the recorded spectrum of the element of body fluid or tissue using a conversion matrix into a standardized spectrum, wherein said conversion matrix has been obtained by calibrating the optical system spectrum response against a spectrum reference previously measured by a reference spectrometer;
• pre-process the converted spectrum by correcting the standardized spectrum for at least scattering;
• correlate, for parameter quantification, the converted pre-processed spectrum with pre-obtained spectral bands for each parameter previously measured by the reference spectrometer;
• wherein said the recorded spectrum is within the ultraviolet—visible—near infrared wavelengths.