Sample preparation method for analysis of platelet proteins
AssigneeRandox Laboratories Limited
InventorsMichael Veitinger, Maria Zellner
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Abstract
The present invention provides a novel method of in vitro sample preparation for the analysis of proteins. In a first aspect, the present invention is a method comprising the separation and individual treatment of blood platelets and platelet-poor plasma obtained from a platelet-rich plasma sample. This allows for optimal treatment of both fractions and overcomes many of the problems associated with current methods. In a second aspect of the present invention the platelet sample preparation method can be implemented before a biochip based immunoassay for the detection of platelet proteins in combination with plasma proteins.
Claims7
A method of preparing an in vitro sample for protein analysis comprising the following steps:
- (i) Centrifuging a platelet suspension and separating the resultant supernatant and pelleted platelets;
- (ii) Separately treating the supernatant and pelleted platelets from step (i) with a lysis buffer or detergent;
- and
- (iii) Combining the resulting lysates of step (ii)
- wherein the platelet suspension is platelet-rich plasma.
Dependencies are inferred from the claim text.