Composition for preparing biomaterial with excellent light-transmitting property, and use thereof
AssigneeRiken
InventorsEtsuo Susaki, Hiroki Ueda, Kazuki Tainaka
Life of this patent
Estimating the term…
Abstract
The present invention provides an aminoalcohol together with, as necessary, at least one selected from the group consisting of: at least one compound selected from the group consisting of urea and a urea derivative; a nonionic detergent; and sucrose to provide a biological material having an excellent light-transmitting property. The present invention provides a technique that allows a high-throughput observation of gene expression/localization or cell morphology in tissue at an whole-tissue level.
Claims6
A method for preparing a biological material having an excellent light-transmitting property,
- the method comprising:
- a first permeating step comprising contacting the biological material with a first solution for a period of time and at a concentration sufficient for permeating the first solution into the biological material and making the biological material having the excellent light-transmitting property,
- wherein the first solution contains
- polyhydric aminoalcohol;
- urea;
- and
- Triton X-100 having a concentration in the range of at least 10% by weight to 20% by weight;
- and
- wherein the excellent light-transmitting property comprises a light transmitting property in which a proportion of transmitted light, with respect to incident light is 40% or more.
A biological material having an excellent light-transmitting property, the biological material comprising
- a biological material that has been permeated with a first solution comprising:
- polyhydric aminoalcohol;
- urea;
- and
- Polyethylene Glycol Mono-p-isooctylphenyl Ether having a concentration in a range of at least 10% by weight to 20% by weight;
- wherein the excellent light-transmitting property comprises a light transmitting property in which a proportion of transmitted light with respect to incident light is 40% or more.
Dependencies are inferred from the claim text.