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US 10,208,356 B1Granted
Massively parallel single cell analysis
AssigneeBecton, Dickinson and Company
InventorsChristina Fan, Stephen P. A. Fodor, Glenn Fu, Geoffrey Richard Facer, Julie Wilhelmy
Life of this patent
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Abstract
The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).
Claims30
A composition comprising:
- a single bead, wherein the bead comprises a plurality of oligonucleotides,
- wherein each of the plurality of oligonucleotides comprises a cellular label sequence, a molecular label sequence, and a target-binding region, wherein the cellular label sequence of each of the plurality of oligonucleotides is the same, wherein the cellular label sequence comprises 4-300 nucleotides, wherein the molecular label sequence comprises 4-300 nucleotides, and at least 100 of the plurality of oligonucleotides comprise different molecular label sequences.
Dependencies are inferred from the claim text.