Methods and probes for monitoring marine water
OwnerHelen Gurney-Smith, Catherine Thomson +1inventors
InventorsHelen Gurney-Smith, Catherine Thomson, Stewart Johnson
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Abstract
A method of biomonitoring marine water is provided, as are probes and kits. The method comprises: extracting messenger Ribonucleic Acid (mRNA) from Mytilus species; preparing cDNA from the mRNA; hybridizing a plurality of probes with the cDNA to provide a set of hybridized cDNA, the probes consisting of at least 20 consecutive nucleotides of at least 50 sequences of SEQ ID NO: 1-122; and quantifying an abundance of the set of hybridized cDNA, thereby biomonitoring marine water.
Claims5
A kit for monitoring environmental quality of marine water, the kit comprising a suitably selected buffer, and a plurality of probes immobilized on a substrate to provide a microarray, the microarray having a set of probes that individually comprise at least 20 consecutive nucleotides of at least SEQ ID NO:
- 1, 3-10, 16 and 30.
Dependencies are inferred from the claim text.