Back to results
US 10,208,343 B2Granted
Methods and systems for processing polynucleotides
Assignee10x Genomics, Inc.
InventorsBenjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol, Xinying Zheng, Phillip Belgrader
Life of this patent
Estimating the term…
Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
Claims32
A library of partitions for barcoding nucleic acid molecules, comprising:
- a plurality of partitions comprising a plurality of gel beads that are degradable upon application of a stimulus, wherein said plurality of gel beads comprises a plurality of barcode sequences that are different across said plurality of gel beads,
- wherein a given partition of said plurality of partitions comprises a given gel bead of said plurality of gel beads and an agent configured to degrade said given gel bead,
- wherein said given gel bead of said plurality of gel beads comprises a plurality of nucleic acid molecules, and
- wherein each of said plurality of nucleic acid molecules comprises a barcode sequence from said plurality of barcode sequences and a unique molecular sequence, wherein (i) said barcode sequence is constant across said plurality of nucleic acid molecules, and (ii) said unique molecular sequence varies across said plurality of nucleic acid molecules.
1 more element
A composition for barcoding a nucleic acid molecule, comprising:
- a partition comprising a bead having a plurality of nucleic acid molecules, wherein each of said plurality of nucleic acid molecules comprises a sequence having a barcode sequence and a unique molecular sequence, wherein (i) said barcode sequence is constant across said plurality of nucleic acid molecules, and (ii) said unique molecular sequence varies across said plurality of nucleic acid molecules, wherein said partition comprises an agent configured to release said sequence into said partition.
Dependencies are inferred from the claim text.