Compositions, devices, systems, and methods for using a nanopore
AssigneeThe Regents of the University of California
InventorsWilliam B. Dunbar, Noah A. Wilson, Mark A. Akeson, David W. Deamer, Kate Lieberman
Life of this patent
Estimating the term…
Abstract
Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
Claims23
A method, comprising:
- providing a device comprising:
- a substrate having a pore therein, the substrate separating a first fluid-filled chamber from a second fluid-filled chamber, wherein the fluid of the first fluid-filled chamber comprises a polynucleotide comprising a macromolecule-binding region and a tethering region;
- and
- a power source electrically coupled to electrodes, wherein the power source and electrodes are adapted to apply a potential difference between the first fluid-filled chamber and the second fluid-filled chamber;
- threading a portion of the polynucleotide through the pore by applying a potential difference between the first fluid-filled chamber and the second fluid-filled chamber;
- and
- tethering the polynucleotide to the pore via the tethering region.
Dependencies are inferred from the claim text.