Use of a porous capillary membrane for determining the amount of rolling circle amplification products
AssigneeVanadis Diagnostics
InventorsOve Öhman, Fredrik Persson, Mathias Howell
Life of this patent
Estimating the term…
Abstract
A method of sample analysis is provided. In certain embodiments, the method may comprise: (a) filtering a liquid sample containing rolling circle amplification (RCA) products using a porous capillary membrane, thereby producing an array of the RCA products on the membrane; wherein the sample contains at least a first population of RCA products and a second population of RCA products, wherein the first and second populations of labeled RCA products are distinguishably labeled; and (b) determining the amount of the first labeled population of RCA products and the amount of the second labeled population of RCA products in an area of the membrane.
Claims20
A method of sample analysis, comprising:
- (a) producing a liquid sample comprising at least a first population of rolling circle amplification (RCA) products and a second population of RCA products, wherein the first and second populations of labeled RCA products are distinguishably labeled and the RCA products are not produced or labeled on or in a porous capillary membrane;
- (b) filtering the liquid sample through a porous capillary membrane, thereby producing an array of the RCA products on the membrane;
- and
- (c) determining the amount of the first labeled population of RCA products and the amount of the second labeled population of RCA products in an area of the membrane.
A method of sample analysis, comprising:
- (a) producing a liquid sample comprising at least a first population of rolling circle amplification (RCA) products and a second population of RCA products, wherein the first and second populations of labeled RCA products are distinguishably labeled and the RCA products are not produced or labeled on or in a capture support;
- (b) filtering the liquid sample through a capture support that acts as a filter for the RCA products, thereby producing an array of the RCA products on the support;
- and
- (c) determining the amount of the first labeled population of RCA products and the amount of the second labeled population of RCA products in an area of the support.
Dependencies are inferred from the claim text.