Sampling devices and methods for concentrating microorganisms

A method for concentrating microorganisms comprising the steps of:

• providing a sampling device comprising
• a unitary first compartment comprising:
• a first reservoir having a first volume;
• a second reservoir having a second volume at least one resealable external opening;
• and
• a passageway connecting the first reservoir to the second reservoir, the passageway having an opening that provides fluid communication between the first reservoir and the second reservoir, wherein the entire first volume is above the opening when the sampling device is in an upright position, wherein the entire second volume is not above the opening when the sampling device is in any position;
• and
• a second component comprising a closure having a protrusion, the closure coupled to the at least one resealable external opening, wherein in a first position the protrusion permits fluid flow through the passageway and wherein in a second position the protrusion prevents fluid flow through the passageway;
• mixing a concentration agent and a sample comprising a microorganism in the unitary sampling device to provide a microorganism bound composition;
• and
• inverting the unitary sampling device such that the second reservoir is oriented substantially above the first reservoir for collecting a major portion of the microorganism bound composition from the second reservoir.