Recombinant microorganism for improved production of fine chemicals
AssigneeBASF SE
InventorsHartwig Schröder, Holger Hartmann, Qingzhao Wang, Shakir Ratani, Zheyuan Guo, Markus Pompejus
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Abstract
The present invention relates to a recombinant nucleic acid molecule, a recombinant microorganism, to a method for producing alanine and to the use of the recombinant nucleic acid molecule or the recombinant microorganism for the fermentative production of alanine.
Claims3
A method of producing pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine comprising culturing a recombinant mocroganism comprising
- (I) a nucleic acid molecule comprising the sequence of SEQ ID NO:
- 49, wherein the codon corresponding to position 43 to 45 of SEQ ID NO:
- 49 does not encode amino acid alanine and is not a stop codon,
- (II) a nucleic acid molecule encoding the polypeptide of SEQ ID NO:
- 50, wherein the amino acid of the polypeptide corresponding to position 15 of SEQ ID NO:
- 50 is not alanine, or
- (III) a nucleic acid molecule encoding a polypeptide having at least 80% identity to the polypeptide of SEQ ID NO:
- 50, wherein the amino acid of the polypeptide corresponding to position 15 of SEQ ID NO:
- 50 is not alanine
- under conditions that allow for the production of pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine.
A process for fermentative production of pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine comprising the steps of
- I) growing, in a fermenter, a microorganism comprising
- (a) a nucleic acid molecule comprising the sequence of SEQ ID NO:
- 49, wherein the codon corresponding to position 43 to 45 of SEQ ID NO:
- 49 does not encode amino acid alanine and is not a stop codon,
- (b) a nucleic acid molecule encoding the polypeptide of SEQ ID NO:
- 50, wherein the amino acid of the polypeptide corresponding to position 15 of SEQ ID NO:
- 50 is not alanine, or
- (c) a nucleic acid molecule encoding a polypeptide having at least 80% identity to the polypeptide of SEQ ID NO:
- 50, wherein the amino acid of the polypeptide corresponding to position 15 of SEQ ID NO:
- 50 is not alanine;
- and
- II) recovering pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine from the fermentation broth obtained in I).
A recombinant microorganism comprising:
- (I) a nucleic acid molecule comprising the sequence of SEQ ID NO:
- 49, wherein the codon corresponding to position 43 to 45 of SEQ ID NO:
- 49 does not encode amino acid alanine and is not a stop codon,
- (II) a nucleic acid molecule encoding the polypeptide of SEQ ID NO:
- 50, wherein the amino acid of the polypeptide corresponding to position 15 of SEQ ID NO:
- 50 is not alanine, or
- (III) a nucleic acid molecule encoding a polypeptide having at least 80% identity to the polypeptide of SEQ ID NO:
- 50, wherein the amino acid of the polypeptide corresponding to position 15 of SEQ ID NO:
- 50 is not alanine
- wherein the microorganism further comprises:
- a) a reduced, repressed or deleted activity and/or expression of a protein encoded by a brnQ gene,
- b) an introduced, increased, or enhanced activity and/or expression of a protein encoded by an argP gene,
- c) an introduced, increased, or enhanced activity and/or expression of a protein encoded by a gcvA gene, and/or
- d) a reduced, repressed or deleted activity and/or expression of a protein encoded by a gcvB gene.
Dependencies are inferred from the claim text.