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US 10,208,320 B2Granted
Primary alcohol producing organisms
AssigneeGenomatica, Inc.
InventorsJun Sun, Anthony P. Burgard, Priti Pharkya
Life of this patent
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Abstract
The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.
Claims19
A non-naturally occurring eukaryotic organism, comprising at least two gene disruptions,
- wherein said at least two gene disruptions comprise a first gene disruption occurring in a gene that encodes an enzyme selected from the group consisting of a cytosolic pyruvate decarboxylase, a mitochondrial pyruvate dehydrogenase, a cytosolic ethanol-specific alcohol dehydrogenase and a mitochondrial ethanol-specific alcohol dehydrogenase, and a second gene disruption occurring in a gene that encodes an enzyme selected from the group consisting of a cytosolic malate dehydrogenase, a glycerol-3-phosphate dehydrogenase shuttle, an external NADH dehydrogenase, and an internal mitochondrial NADH dehydrogenase, and
- wherein said at least two gene disruptions confers production of long chain alcohols in the cytosol of said organism.
Dependencies are inferred from the claim text.