RNA-YY1 interactions

A method of identifying a compound that disrupts binding of a Yin-Yang 1 (YY1)-binding long non-coding RNA (lncRNA) to Yin-Yang 1 (YY1) protein, the method comprising:

• (a) identifying an lncRNA that binds YY1 protein;
• (b) providing a cell-free sample comprising a YY1-binding lncRNA and YY1 protein, wherein the YY1-binding lncRNA binds to the YY1 protein thereby forming lncRNA-YY1 protein complexes;
• (c) contacting the cell-free sample with a test compound, wherein the test compound is a nucleic acid targeting the YY1-binding lncRNA;
• (d) isolating the lncRNA-YY1 protein complexes from the cell-free sample by contacting the cell-free sample comprising the lncRNA-YY1 protein complexes with an anti-YY1 antibody, wherein the anti-YY1 antibody specifically binds to YY1 protein, and immunoprecipitating the lncRNA-YY1 protein complexes;
• and
• (e) detecting formation of the lncRNA-YY1 protein complexes in presence and absence of the test compound, wherein a decrease in formation of lncRNA-YY1 protein complexes in the presence of the test compound as compared to formation of lncRNA-YY1 protein complexes in the absence of test compound indicates that the test compound disrupts the binding of the YY1-binding lncRNA to the YY1 protein.