Adenovirus library and methods
AssigneeRegents of the University of Minnesota
InventorsMasato Yamamoto, Yoshiaki Miura
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Abstract
Described herein is a method that generally includes infecting a host cell with a rescue adenovirus, wherein the rescue adenovirus genome comprises a loxP site and encodes at least one marker, and wherein the host cell comprises a library of polynucleotides that complement the adenovirus genome marker and encode a detectable polypeptide; incubating the infected host cell under conditions effective to permit recombination between the adenovirus genome and one or more of the library polynucleotides and the production of recombinant adenovirus particles comprising at least on detectable polypeptide; and detecting the at least one detectable polypeptide. Also described are adenovirus libraries constructed using such a method.
Claims9
A method comprising:
- infecting fiber transcomplementing mammalian host cells with an Ad5/3 pseudotyped, genetically fiberless rescue adenovirus, wherein the rescue adenovirus genome comprises a single loxP site, the host cells modified to express CRE recombinase;
- transfecting the host cells with a library of plasmids, each plasmid:
- comprising a loxP site;
- and
- encoding at least one detectable polypeptide comprising a fiber polypeptide comprising an AB-loop comprising a targeting ligand, wherein the library of plasmids comprises a plurality of detectable polypeptides;
- incubating the infected and transfected host cell under conditions effective to permit:
- recombination between the adenovirus genome and the plasmid, and
- production of recombinant adenovirus particles comprising at least one detectable polypeptide;
- and
- detecting the at least one detectable polypeptide.
Dependencies are inferred from the claim text.