Compositions and methods for the biosynthesis of vanillan or vanillin beta-D-glucoside
AssigneesInternational Flavors & Fragrances Inc., Evolva SA
InventorsJoergen Hansen, Esben Halkjaer Hansen, Honey Polur, Joseph M. Sheridan, Jonathan R. Heal, William D. O. Hamilton
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Abstract
Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express a mutant AROM polypeptide and/or mutant catechol-O-methyltransferase polypeptide alone or in combination with one or more vanillin biosynthetic enzymes or UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce vanillin or vanillin beta-D-glucoside.
Claims2
A recombinant mutant Arom Multifunctional Enzyme (AROM) polypeptide having the amino acid sequence of SEQ ID NO:20 or SEQ ID NO:22.
Dependencies are inferred from the claim text.