US 10,208,290 B2 · Virion display array for profiling functions and interactions of human membrane proteins

Abstract

Provided herein are recombinant virion arrays comprising human membrane bound proteins that retain their native conformations and/or interactions, recombinant HSV-1 virions, and methods of use including high-content, high-throughput assays for screening for ligands and/or drugs that bind human membrane bound proteins, diagnostic assays, proteomic assays, and biosensor assays. Also provided are recombinant HSV-1 virions comprising an envelope comprising a plurality of heterologous membrane bound proteins that retain their native conformations and/or interactions as well as recombinant HSV-1 bacterial artificial chromosome (BAC) clones encoding heterologous membrane polypeptides.

Claims25

Dependent claims

An array comprising:

(a) a substrate;
and
(b) a plurality of recombinant herpesviridae virion microspots associated with a surface of the substrate, wherein the recombinant herpesviridae virion microspots comprise a plurality of recombinant herpesviridae virions, wherein the recombinant herpesviridae virions comprise envelopes comprising a plurality of human heterologous membrane bound proteins that retain their native conformations, interactions, or both, and wherein the human heterologous membrane bound proteins are encoded by the genomes of the recombinant herpesviridae virions.

Dependencies are inferred from the claim text.

Facts

Application

14/900,314Filed 20 Jun 2014 · us provisional application

Priority

21 Jun 2013

Published

US 2016/0355789 A18 Dec 2016

Granted

19 Feb 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification19 codes

A61B 18/02
Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body: by cooling, e.g. cryogenic techniques
G01N 33/6854
Involving proteins, peptides or amino acids: immunoglobulins
A61B 17/3478
Trocars; Puncturing needles: endoscopic needles, e.g. for infusion
A61B 18/0218
By cooling, e.g. cryogenic techniques: with open-end cryogenic probe, e.g. for spraying fluid directly on tissue or via a tissue-contacting porous tip

Virion display array for profiling functions and interactions of human membrane proteins

Life of this patent

Virion display array for profiling functions and interactions of human membrane proteins

Life of this patent

Abstract

Claims25