CD82-positive cardiac progenitor cells
AssigneeIheart Japan Corporation
InventorsJun Yamashita, Masafumi Takeda
Life of this patent
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Abstract
An object of the present invention is to provide a myocardial progenitor cell that is specifically induced to differentiate into a cardiomyocyte, and a method for preparing the myocardial progenitor cell. A method for preparing a CD82-positive cell includes, in sequence, a step (a) of obtaining stem cells, a step (b) of subjecting the stem cells to induction treatment of differentiation into cardiovascular cells, and a step (c) of separating, from the stem cells having been subjected to the induction treatment of differentiation in the step (b), a CD82-positive cell being negative for at least one cell surface marker selected from CD73, CD44, CD105, CD121a, CD18, and CD120a. This method enables preparation of a CD82-positive cell for use as a myocardial progenitor cell.
Claims19
A composition comprising:
- a cell population containing a myocardial progenitor cell that is CD82-positive, and is negative for at least one cell surface marker selected from a group consisting of CD73, CD44, CD105, CD121a, CD18, and CD120a, and
- a physiological aqueous solution,
- wherein the myocardial progenitor cell is contained in the cell population at a ratio of at least 80%, and
- wherein the physiological aqueous solution contains a cryoprotectant.
A method for preparing a myocardial progenitor cell, comprising steps (a) to (c) below,
- (a) a step of obtaining pluripotent stem cells;
- (b) a step of subjecting the pluripotent stem cells to induction treatment of differentiation into cardiovascular cells;
- and
- (c) a step of isolating, from the pluripotent stem cells having been subjected to the induction treatment of differentiation in the step (b), a myocardial progenitor cell that is CD82-positive, and is negative for at least one cell surface marker selected from a group consisting of CD73, CD44, CD105, CD121a, CD18, and CD120a, using an anti-CD82 antibody.
Dependencies are inferred from the claim text.