Cell-seeding and -culturing device

A cell seeding and culturing device capable of forming a nerve cell network, comprising:

• a cell culturing substrate having a plurality of cell arrangement areas surrounded by a plurality of projections, which inhibit cell moving and loss, and enables nerve cells to form a nerve cell network between the cell arrangement areas;
• a removable flow channel substrate having a plurality of through holes through which cells are allowed to pass, and being arranged on the culturing substrate, wherein each of the through holes defines a flow channel, wherein the upper side of the removable flow channel substrate is an entrance and the lower side of the removable flow channel substrate is an exit, wherein the flow channel is tapered from an entrance to an exit so as to seed cells, the exit of each flow channel is located above the cell arrangement areas, and the size of the exit is smaller than that of the cell arrangement area;
• and
• a spacer member having a thickness greater than the height of the projections, which is arranged between the culturing substrate and the removable flow channel substrate, wherein a gap which does not allow cells to flow out is formed between the exits of the flow channels and the projections.