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US 10,208,283 B2Granted
Methods and systems for manipulating particles using a fluidized bed
AssigneeSartorius Stedim North America Inc.
InventorsSunil Mehta, Tod Herman, Harold Ross, Khurshid Iqbal, Joe McMahon
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Abstract
The present invention comprises methods and systems for manipulation of media and particles, whether inert materials or biomaterials, such as cells in suspension cell culture. The methods and systems comprise use of an apparatus comprising a rotating chamber wherein the actions of the combined forces of gravity, fluid flow force and centrifugal force form a fluidized bed within the rotating chamber.
Claims19
A method for manipulating particles using a fluidized bed, the method comprising:
- rotating a chamber about a substantially horizontal axis to create a centrifugal force field, the chamber comprising an inlet and an outlet;
- flowing a first stream containing a first media and particles into the chamber through the inlet, wherein flowing the first stream acts to create a force which opposes the centrifugal force;
- forming a fluidized bed of particles in the chamber, wherein the forces substantially immobilize the particles in the fluidized bed by the summation of vector forces acting on the particles;
- collecting the first media substantially without particles passing through the outlet of the chamber;
- then
- manipulating the particles in the fluidized bed, wherein said manipulating is selected from the group consisting of removing, concentrating, diluting, exchanging media, harvesting, transferring, dispensing, transfecting, electroporating, separating, extracting, isolating, selecting, purifying, coating, binding, physically modifying, and altering the environment;
- and thereafter
- removing the particles from the fluidized bed, wherein removing the particles comprises:
- flowing a second stream into the chamber through the outlet, wherein flowing the second stream acts to create a force at least partially in the same direction as the centrifugal force field;
- and
- collecting the particles passing through the inlet of the chamber.
8 more elements
A method for separating a mixed population of particles, the method comprising:
- rotating a chamber about a substantially horizontal axis, the chamber having an inlet and an outlet;
- substantially immobilizing an affinity matrix in the chamber by forming a fluidized bed of the affinity matrix in the chamber;
- flowing a first stream containing a first media and a mixed population of particles comprising target particles and non-target particles into the chamber through the inlet;
- retaining target particles in the affinity matrix in the chamber;
- and
- collecting the first media and non-target particles passing through the outlet of the chamber.
3 more elements
A method for fractionating biomaterials, the method comprising:
- rotating a chamber about a substantially horizontal axis to create a centrifugal force field, the chamber having an inlet and an outlet;
- flowing a first stream containing a first media and a mixture of biomaterials into the chamber through the inlet, wherein flowing the first stream acts to create a force which opposes the centrifugal force;
- selectively precipitating biomaterials from the first stream;
- forming a fluidized bed of the precipitated biomaterials in the chamber, wherein the forces substantially immobilize the precipitated biomaterials in the fluidized bed by the summation of vector forces acting on the precipitated biomaterials;
- then
- collecting the first media and the non-precipitated biomaterials passing through the outlet of the chamber;
- and thereafter
- removing the precipitated biomaterials from the fluidized bed, wherein removing the precipitated biomaterials comprises:
- flowing a second stream into the chamber through the outlet, wherein flowing the second stream acts to create a force at least partially in the same direction as the centrifugal force field;
- and
- collecting the precipitated biomaterials passing through the inlet of the chamber.
8 more elements
A method for separating a mixed population of particles, the method comprising:
- rotating a chamber about a substantially horizontal axis, the chamber having an inlet and an outlet;
- substantially immobilizing an affinity matrix in the chamber;
- flowing a first stream containing a first media and a mixed population of particles comprising target particles and non-target particles from a separate non-rotating container into the chamber through the inlet while the chamber is rotating;
- retaining target particles in the affinity matrix in the chamber;
- and
- collecting the first media and non-target particles passing through the outlet of the chamber.
3 more elements
Dependencies are inferred from the claim text.