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US 10,208,142 B2Granted
Polymers and DNA copolymer coatings
AssigneeIllumnia Cambridge Limited
InventorsAndrew A. Brown, Wayne N. George, Alexandre Richez, Anne-Cecile Dingwall, Xavier von Hatten
Life of this patent
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Abstract
Some embodiments described herein relate to new polymer coatings for surface functionalization and new processes for grafting pre-grafted DNA-copolymers to surface(s) of substrates for use in DNA sequencing and other diagnostic applications.
Claims22
A substrate having a first surface comprising a polymer for surface functionalization covalently bonded thereto, wherein the polymer comprises a recurring unit of Formula (I) and a recurring unit of Formula (II):
- wherein:
- each of R1a, R2a, R1b and R2b is independently selected from the group consisting of hydrogen and optionally substituted alkyl;
- each of R3a and R3b is independently selected from the group consisting of hydrogen, optionally substituted alkyl, optionally substituted phenyl, and optionally substituted C7-14 aralkyl;
- and
- each of L1 and L2 is independently selected from the group consisting of an optionally substituted alkylene linker and an optionally substituted heteroalkylene linker.
2 more elements
A method of immobilizing a polymer to a first surface of a substrate comprising:
- contacting a polymer comprising a recurring unit of Formula (I) and a recurring unit of Formula (II) with the first surface of the substrate, said first surface comprising a first plurality of functional groups covalently attached thereto;
- and
- reacting the first plurality of functional groups of the first surface with the polymer, thereby covalently bonding the polymer to the first surface of the substrate;
- wherein:
- each of R1a, R2a, R1b and R2b is independently selected from the group consisting of hydrogen and optionally substituted alkyl;
- each of R3a and R3b is independently selected from the group consisting of hydrogen, optionally substituted alkyl, optionally substituted phenyl, and optionally substituted C7-14 aralkyl;
- and
- each of L1 and L2 is independently selected from the group consisting of an optionally substituted alkylene linker and an optionally substituted heteroalkylene linker.
5 more elements
Dependencies are inferred from the claim text.