Compositions and methods related to recombinant antibodies to histone posttranslational modifications
AssigneeThe University of Chicago
InventorsTakamitsu Hattori, Shohei Koide, Joseph Taft, Akiko Koide
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Abstract
Embodiments concern compositions and methods involving recombinant antibodies to histone post-translational modifications. The invention provides compositions and methods for histone methyltransferase assays. In certain embodiments, the compositions and methods involve a recombinant antibody that binds histone H3 fragment harboring biomarkers such as H3K9me3 mark, H3K4me3 mark, H3K36me3 mark, H3K27me3, H3K9me3 and H3S10phos or a recombinant antibody that binds histone H4 fragment harboring H4K20me3 mark.
Claims1
A purified recombinant polypeptide comprising a variable heavy chain region comprising the sequence of complementarity determining regions (CDR) CDR-H1, CDR-H2, and CDR-H3 of the 309M3-B antibody and a variable light chain region comprising the sequence of complementarity determining regions CDR-L1, CDR-L2, and CDR-L3 of the 309M3-B antibody, wherein the polypeptide bind to H3K9me3.
Dependencies are inferred from the claim text.