Recombinant fibrinogen high-production line and method for producing same
AssigneeJapan Blood Products Organization
InventorsShusei Uno, Momoko Otaki, Kouji Murakami, Shoji Ideno
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Abstract
The present invention provides a recombinant strain highly producing fibrinogen which is an animal cell strain coexpressing a fibrinogen and an α2PI and/or PAI-2, genes encoding Aα chain, Bβ chain and γ chain of fibrinogen, a production method of a recombinant strain highly producing fibrinogen, including introducing gene(s) encoding α2PI and/or PAI-2 into an animal cell, and coexpressing fibrinogen and α2PI and/or PAI-2 in the animal cell, and a production method of a recombinant fibrinogen including culturing a recombinant strain highly producing fibrinogen in a medium, and recovering fibrinogen from the obtained culture.
Claims10
A recombinant animal cell comprising genes encoding the Aα chain, Bβ chain, and γ chain of fibrinogen and gene(s) encoding α2 plasmin inhibitor (α2PI) and/or plasminogen activator inhibitor-2 (PAI-2), wherein functional fibrinogen is produced by coexpressing said fibrinogen genes with α2PI and/or PAI-2 in said animal cell, and wherein said cell suppresses degradation of fibrinogen during cell culture and/or increases the production amount of fibrinogen independent of the suppressive effect, compared to the same animal cell expressing fibrinogen but which does not coexpress α2PI and/or PAI-2.
A method of making a recombinant animal cell that produces fibrinogen, comprising introducing genes encoding Aα chain, Bβ chain and γ chain of fibrinogen, and gene(s) encoding a2 plasmin inhibitor (a2PI) and/or plasminogen activator inhibitor-2 (PAI-2) into an animal cell, and coexpressing the fibrinogen and a2PI and/or PAI-2 in the animal cell, wherein said cell suppresses degradation of fibrinogen during cell culture and/or increases the production amount of fibrinogen independent of the suppressive effect, compared to an animal cell expressing fibrinogen but which does not coexpress α2PI and/or PAI-2.
Dependencies are inferred from the claim text.