Plasmid, method and kit thereof for producing heat labile enterotoxin B-subunit
AssigneeAgricultural Technology Research Institute
InventorsJiunn-Horng Lin, Jyh-Perng Wang, Chien-Yu Fang, Zeng-Weng Chen, Ming-Wei Hsieh, Hao-Zhen Zeng, Jian-Fong Lai, Weng-Zeng Huang, Tzu-Ting Peng
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Abstract
The present invention provides a plasmid, method and kit for producing heat labile enterotoxin B-subunit based on a Bacillus subtilis expression system. By comparing with the conventional method in the art, the present invention has the advantages of high safety, good yield, and simplified process and is therefore favorable for the commercialization object of heat labile enterotoxin B-subunit in the application of vaccine adjuvant.
Claims22
A plasmid for producing Escherichia coli heat labile enterotoxin B-subunit, comprising:
- a nucleotide sequence encoding Escherichia coli heat labile enterotoxin B-subunit;
- and
- an expression element recognizable by Bacillus subtilis;
- wherein said expression element is rpsJ-lacO expression element, veg6-lacO expression element, or a combination thereof.
Dependencies are inferred from the claim text.