Microfluidic device for detecting cells of blood

A microfluidic device comprising:

• a substrate having at least one wandering groove formed on a first surface thereof;
• a shielding member abutting on the first surface of the substrate and covering the groove to form a flow channel to deliver blood between the substrate and the shielding member;
• an inlet at one end of the flow channel for introducing the blood into the flow channel;
• and
• an outlet at another end of the flow channel for discharging the blood;
• wherein
• the flow channel has a detecting region near the outlet for cells of the blood passing through separately, the detecting region includes a narrowed channel, an entry for a single cell entering into the narrowed channel at a time and an exit for a single cell exiting out the narrowed channel at a time;
• and at least a pair of electrodes are located in the narrowed channel and disposed between the entry and the exit to detect the cells of the blood;
• the shielding member comprises a cover, and a double side tape having a first side sticking on a surface of the cover and an opposite second side sticking on the first surface of the substrate;
• and
• the electrodes are plated on the surface of the cover using laser direct structuring (LDS) technology, and the double side tape has a pair of holes aligning to the electrodes respectively to expose the electrodes to the detecting region of the flow channel.