Single-pass filtration systems and processes
AssigneeEMD Millipore Corporation
InventorsHerbert Lutz, Joseph Parrella, Bala Raghunath
Life of this patent
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Abstract
The present invention provides, in various embodiments, hybrid single-pass tangential flow filtration assemblies, disposable single-pass tangential flow filtration assemblies, scalable single-pass tangential flow filtration assemblies and adaptable modular single-pass tangential flow filtration assemblies. In other embodiments, the invention relates to processes for recovering proteins from the surface of a filtration membrane in a single-pass tangential flow filtration assembly and for cleaning a tangential flow filtration assembly. In additional embodiments, the invention provides methods of increasing the processing capacity of a single-pass tangential flow filtration assembly.
Claims21
A single-pass tangential flow filtration (SPTFF) assembly, comprising:
- a plurality of stacked processing units that are fluidly connected, wherein each processing unit comprises a cassette holder containing a first channel, a second channel and a permeate channel and at least one TFF cassette, and wherein the processing units in the assembly are configured for processing in parallel and/or in series;
- wherein the first channel, the second channel and the permeate channel of adjacent stacked cassette holders in the processing units are fluidly aligned such that
- the first channel connected to a feed inlet extends through each cassette holder of the plurality of processing units;
- the second channel connected to a retentate outlet extends through each cassette holder of the plurality of processing units;
- the permeate channel extends through each cassette holder of the plurality of processing units;
- and
- one or more valves, wherein each valve is located on either the first channel or the second channel between two adjacent cassette holders in the processing units, and wherein the valves alternate between the first channel and second channel for consecutive pairs of cassette holders in adjacent processing units;
- wherein the plurality of processing units are processed in parallel when the valves are open, or in series when the valves are closed.
A tangential flow filtration (TFF) assembly, comprising:
- a plurality of stacked processing units that are fluidly connected, wherein each processing unit comprises a cassette holder containing a first channel, a second channel and a permeate channel and at least one TFF cassette, and wherein the processing units in the assembly are configured for processing in parallel and/or in series;
- wherein the first channel, the second channel and the permeate channel of adjacent stacked cassette holders in the processing units are fluidly aligned such that
- the first channel connected to a feed inlet extends through each cassette holder of the plurality of processing units;
- the second channel connected to a retentate outlet extends through each cassette holder of the plurality of processing units;
- the permeate channel extends through each cassette holder of the plurality of processing units;
- at least one pump or control valve for recirculating retentate through all or part of the assembly;
- at least one conduit for recirculating retentate;
- and
- one or more valves, wherein each valve is located on either the first channel or the second channel between two adjacent cassette holders in the processing units, and wherein the valves alternate between the first channel and second channel for consecutive pairs of cassette holders in adjacent processing units;
- wherein the plurality of processing units are processed in parallel when the valves are open, or in series when the valves are closed.
Dependencies are inferred from the claim text.