Method for cellular RNA expression
AssigneesTron Translationale Onkologie An Der Universitatsm, BioNTech RNA Pharmaceuticals GmbH
InventorsUgur Sahin, Tim Beissert, Marco Poleganov, Stephanie Herz, Lars Koste
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Abstract
The present invention relates to expressing RNA in cells and, in particular, enhancing viability of cells in which RNA is to be expressed. Specifically, the present invention provides methods for expressing RNA in cells comprising the steps of preventing engagement of IFN receptor by extracellular IFN and inhibiting intracellular IFN signalling in the cells. Thus, preventing engagement of IFN receptor by extracellular IFN and inhibiting intracellular IFN signalling in the cells allows repetitive transfer of RNA into the cells.
Claims33
A method for expressing a first RNA in a cell comprising the steps of (i) preventing engagement of IFN receptor by extracellular IFN by providing vaccinia virus B18R to the cell in the form of second RNA encoding vaccinia virus B18R and (ii) inhibiting intracellular IFN signaling by providing vaccinia virus E3 to the cell in the form of third RNA encoding vaccinia virus E3 and by providing vaccinia K3 to the cell in the form of fourth RNA encoding vaccinia virus K3;
- wherein the ribonucleotides of the first RNA comprise naturally occurring nucleotides;
- and wherein the second, third and
- fourth RNAs are simultaneously introduced into the cell.
Dependencies are inferred from the claim text.