US 10,206,994 B2 · RNA virus attenuation by alteration of mutational robustness and sequence space

Abstract

The application generally relates to the attenuation of a RNA virus or of a clone thereof and involves the alteration of sequence space, more particularly the reduction, of mutational robustness of said RNA virus or clone. The means of the application are more particularly dedicated to the attenuation of an infectious RNA virus or clone, for the production of immunogenic composition or vaccine. More particularly, the means of the application involve the replacement of codon(s) by different codon(s), which is(are) selected to differ by only one nucleotide from a codon STOP, more particularly by different but synonymous codon(s), which is(are) selected to differ by only one nucleotide from a codon STOP.

Claims28

Dependent claims

A process for producing an attenuated RNA virus or a cDNA clone thereof comprising a RNA-dependent RNA polymerase or a RNA-dependent DNA polymerase, the process comprising:

providing an infectious RNA virus or a cDNA clone thereof comprising the retrotranscript of the CDS of the genome of the RNA virus;
and
modifying the RNA genome of the infectious RNA virus or the retrotranscript of the CDS of the genome of the RNA virus, respectively;
wherein the modification comprises changing at least one codon that codes for an amino acid selected from Leu, Ser, Arg and Gly in the infectious RNA virus or cDNA clone thereof to a different but synonymous codon,
wherein said different but synonymous codon differs by only one nucleotide from a STOP codon.

2 more elements

Dependencies are inferred from the claim text.

RNA virus attenuation by alteration of mutational robustness and sequence space

Life of this patent

RNA virus attenuation by alteration of mutational robustness and sequence space

Life of this patent

Abstract

Claims28