Stabilisation of viral particles
AssigneeStabilitech Biopharma Ltd
InventorsJeffrey Drew, David Woodward, John Bainbridge, Amanda Corteyn
Life of this patent
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Abstract
A method for preserving viral particles comprising: (a) providing an aqueous solution of (i) viral particles, (ii) optionally one or more sugars, and (iii) a compound of formula (I) or a physiologically acceptable salt or ester thereof and/or a compound of formula (II) or a physiologically acceptable salt or ester thereof; and (b) drying the solution to form a composition incorporating said viral particles.
Claims13
A method for preserving live viral particles during freeze-drying, the method comprising:
- (a) preparing an aqueous solution, which is optionally buffered, the solution comprising:
- (i) live viral particles selected from Adenoviridae, Orthomyxoviridae, Paramyxoviridae, Parvoviridae, Picornaviridae, Poxviridae, Calciviridae, Coronaviridae, Filoviridae, Flaviviridae, Hepadnaviridae, Herpesviridae, Papillomaviridae, Reoviridae, Retroviridae, and Toqaviridae, and
- (ii) as the sole excipients one or more sugars at a total sugar concentration of 0.1 M to 3M, 0.1 to 1.5M of N,N-dimethylglycine or a physiologically acceptable salt or ester thereof, and 0.1 to 1.5M of methylsulfonylmethane;
- and
- (b) freeze-drying the solution prepared in step (a) to form a composition incorporating said live viral particles.
A method of preparing a vaccine which incorporates live viral particles that are preserved during freeze-drying, which method comprises:
- (a) preparing an aqueous solution, which is optionally buffered, the solution comprising:
- (i) live viral particles selected from Adenoviridae, Orthomyxoviridae, Paramyxoviridae, Parvoviridae, Picornaviridae, Poxviridae, Calciviridae, Coronaviridae, Filoviridae, Flaviviridae, Hepadnaviridae, Herpesviridae, Papillomaviridae, Reoviridae, Retroviridae, and Togaviridae, and
- (ii) as the sole excipients one or more sugars at a total sugar concentration of 0.1 M to 3M, 0.1 to 1.5M of N,N-dimethylglycine or a physiologically acceptable salt or ester thereof, and 0.1 to 1.5M of methylsulfonylmethane;
- and
- (b) optionally adding an adjuvant, buffer, antibiotic and/or additive to the aqueous solution of (a);
- and
- (c) freeze-drying the solution prepared in step (a) or (b) to form a solid composition incorporating said live viral particles.
A method for preserving live viral particles during freeze-drying, the method comprising:
- (a) preparing an aqueous solution which consists of:
- (i) live viral particles selected from Adenoviridae, Orthornyxoviridae, Paramyxoviridae, Parvoviridae, Picornaviridae, Poxviridae, Calciviridae, Coronaviridae, Filoviridae, Flaviviridae, Hepadnaviridae, Herpesviridae, Papillomaviridae, Reoviridae, Retroviridae, and Togaviridae,
- (ii) one or more sugars at a total sugar concentration of 0.1M to 3M,
- (iii) 0.1 to 1.5M of N,N-dimethylglycine or a physiologically acceptable salt or ester thereof,
- (iv) 0.1 to 1.5M of methylsulfonylmethane;
- (iv) optionally one or more buffers;
- and
- (b) freeze-drying the solution prepared in step (a) to form a composition incorporating said live viral particles.
A method of preparing a vaccine which incorporates live viral particles that are preserved during freeze-drying, which method comprises:
- (a) preparing an aqueous solution, which is optionally buffered, and consists of (i) live viral particles selected from Adenoviridae, Orthomyxoviridae, Paramyxoviridae, Parvoviridae, Picornaviridae, Poxviridae, Calciviridae, Coronaviridae, Filoviridae, Flaviviridae, Hepadnaviridae, Herpesviridae, Papillornaviridae, Reoviridae, Retroviridae, and Toqaviridae, (ii) one or more sugars at a total sugar concentration of 0.1M to 3M, (iii) 0.1 to 1.5M of N,N-dimethylglycine or a physiologically acceptable salt or ester thereof, and (iv) 0.1 to 1.5M of methylsulfonylmethane;
- and
- (b) optionally adding an adjuvant, buffer, antibiotic and/or additive to the aqueous solution of (a);
- and
- (c) freeze-drying the solution prepared in step (a) or (b) to form a composition or solid composition incorporating said live viral particles.
Dependencies are inferred from the claim text.