Preparation of a cell concentrate from a physiological solution
AssigneesSmith & Nephew, Inc., Pall Corporation
InventorsSamuel O. Sowemimo-Coker, Marcus Lee Scott, Marc Long, Ed Margerrison, Michael B. Cooper
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Abstract
The present invention is directed to methods and compositions regarding the preparation of an cell concentrate, such as, for example, an osteogenic cell concentrate, from a physiological solution, such as bone marrow aspirate, blood, or a mixture thereof. In specific embodiments, the invention provides methods and compositions utilizing two physiological solution-processing techniques, particularly in a point of care environment, wherein centrifugation is not employed.
Claims36
A method of preparing a cell concentrate, comprising the steps of:
- providing a physiological fluid not previously subjected to centrifugation;
- subjecting said physiological fluid to a filter to produce a filter retentate and a permeate fluid, wherein said filter retentate comprises a cell concentrate including both platelets and nucleated cells per unit volume greater than in the physiological fluid, wherein the nucleated cells comprise leukocytes, and wherein said permeate fluid comprises plasma and red blood cells, and wherein the filter comprises a leukocyte reduction filter;
- removing the cell concentrate from the filter;
- and
- delivering the cell concentrate to a bone defect in an individual.
A method of preparing a a scaffold material/filter retentate mixture, comprising the steps of:
- providing a physiological fluid not previously subjected to centrifugation;
- subjecting said physiological fluid to a filter to produce a filter retentate and a permeate fluid, wherein said filter retentate comprises platelets and nucleated cells per unit volume greater than in the physiological fluid wherein the nucleated cells comprise leukocyes and wherein said permeate fluid comprises plasma and red blood cells, and wherein the filter comprises a leukocyte reduction filter;
- removing the filter retentate from the filter;
- and
- admixing a scaffold material to the filter retentate removed from the filter to produce a scaffold material/filter retentate mixture.
Dependencies are inferred from the claim text.