Syringe-based fluid diversion mechanism for bodily fluid sampling

A method of sequestering bodily fluid withdrawn from a patient with a bodily fluid sequestration device, the method comprising:

• establishing fluid communication between the patient and a channel of the bodily fluid sequestration device via a port, the channel defining a first fluid flow path and including a valve configured to form a substantially fluid tight seal with walls defining the channel;
• receiving, without moving at least a portion of the valve relative to the port, an initial volume of bodily fluid from the patient into a pre-sample reservoir of the bodily fluid sequestration device via the port and the first fluid flow path, the first fluid flow path extending from a proximal end of the port when the initial volume of bodily fluid is received;
• sequestering the initial volume of bodily fluid in the pre-sample reservoir;
• after the initial volume of bodily fluid is sequestered, receiving a subsequent volume of bodily fluid from the patient via a second fluid flow path of the bodily fluid sequestration device, at least a portion of the second fluid flow path formed separately from the first fluid flow path;
• the valve being operative to move from a closed configuration to an open configuration in response to a difference in pressure between a valve inlet and a valve outlet,
• the valve being further operative to return to the closed configuration in response to equalization of pressure between the valve inlet and the valve outlet, which is configured to allow the creation of a negative pressure in the second fluid flow path operable to draw the subsequent volume of bodily fluid from the patient;
• establishing fluid communication with a sample vessel containing a culture medium;
• and
• providing via the second fluid flow path a volume of bodily fluid into the sample vessel, the volume of bodily fluid having reduced contamination due to the sequestration of the initial volume of bodily fluid.