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US 10,202,638 B2Granted
Compositions and methods for performing hybridizations with separate denaturation of the sample and probe
AssigneeDako Denmark A/S
InventorSteen Hauge Matthiesen
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Abstract
The invention provides methods and compositions for separately denaturing a probe and target in hybridization applications. The invention may, for example, eliminate the use of or reduce the dependence on formamide in hybridization applications. Compositions for use in the invention include an aqueous composition comprising at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.
Claims20
A method of hybridizing nucleic acid sequences comprising:
- combining a first nucleic acid sequence with a first aqueous composition comprising at least one polar aprotic solvent in an amount effective to denature a double-stranded nucleotide sequence, and
- combining said first nucleic acid sequence with a second nucleic acid sequence for at least a time period sufficient to hybridize the first and second nucleic acid sequences,
- wherein the polar aprotic solvent is not dimethyl sulfoxide (DMSO).
Dependencies are inferred from the claim text.