Hepatitis B virus capsid assembly
AssigneeThe Penn State Research Foundation
InventorJianming Hu
Life of this patent
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Abstract
Cell-free translation and assembly systems allow for HBV capsid assembly under cell-free conditions that also mimic the physiological salt and protein concentrations. These hepatitis virus capsid assembly systems utilize the C-terminal domain (CTD) and N-terminal domain (NTD) of the hepatitis capsid protein in capsid assembly. Uses of the system include the identification of potential new therapeutic strategies and screening of potential therapeutic agents which target these domains and modulate capsid assembly and/or disassembly and other functions of these domains in HBV replication and pathogenesis.
Claims4
A method for assembling Hepatitis virus capsids in vitro comprising:
- transcribing, translating or expressing one or more hepatitis virus capsid or core protein nucleic acids, polynucleotides, oligonucleotides, proteins, peptides, mutants or fragments thereof, which encodes for or comprises a hepatitis virus capsid or core protein C-terminal Domain (CTD) and N-terminal domain (NTD) sufficient to form capsids, in a rabbit reticulocyte lysate (RRL) cell-free system, said RRL cell-free system comprising at least one host factor for controlling a phosphorylation state of a capsid protein, said at least one host factor being Cyclin-dependent kinase 2 (CDK2) or Protein phosphatase 2 (PP2A).
Dependencies are inferred from the claim text.