Nicotiana benthamiana plants deficient in fucosyltransferase activity
AssigneeIcon Genetics GmbH
InventorsKoen Weterings, Gerben Van Eldik
Life of this patent
Estimating the term…
Abstract
The invention provides methods for reducing the levels of alfa (1,3)-fucosylated N-glycans on glycoproteins produced in plants or plant cells. In addition, the invention provides alfa(1,3)-fucosyltransferase genes from Nicotiana benthamiana, and mutant N. benthamiana plants in which the levels of alfa(1,3)-fucosylated N-glycans are reduced.
Claims8
A Nicotiana benthamiana plant, cell, part, seed, or progeny thereof, comprising at least three knock-out alfa(1,3)-fucosyltransferase genes, wherein one or more of the knock-out alfa(1,3)-fucosyltransferase genes is a mutated version of a native alfa(1,3)-fucosyltransferase gene selected from the group consisting of:
- a. FucTA gene containing a G to A substitution at position 355 of SEQ ID NO:
- 1;
- b. FucTB gene containing a G to A substitution at position 3054 of SEQ ID NO:
- 4;
- c. FucTC gene containing a G to A substitution at position 2807 of SEQ ID NO:
- 7;
- d. FucTD gene containing a G to A substitution at position 224 of SEQ ID NO:
- 10;
- and
- e. FucTE gene containing a G to A substitution at position 910 of SEQ ID NO:
- 13.
Dependencies are inferred from the claim text.