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AssigneeEvonik Degussa GmbH
InventorsWanda Dischert, Perrine Vasseur, Rainer Figge
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The present application is related to a recombinant microorganism optimized for the fermentative production of methionine and/or its derivatives, wherein in said recombinant microorganism, the cobalamin-dependent methionine synthase activity and the methionine efflux are enhanced. The application is also related to a method for optimizing the fermentative production of methionine and/or its derivatives comprising the steps of: c. culturing a recombinant microorganism wherein in said microorganism, the cobalamin-dependent methionine synthase activity and the methionine efflux are enhanced, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and d. recovering methionine and/or its derivatives from the culture medium.
A recombinant microorganism genetically modified to improve the production of methionine in comparison with an endogenous production of methionine in the corresponding wild-type microorganism, wherein said recombinant microorganism is recombinant E. coli and wherein in said recombinant microorganism, the expression of the E. coli metH, E. coli fldA and E. coli fpr genes is enhanced compared to the expression of said genes in the corresponding unmodified microorganism and the E. coli ygaZH genes, Citrobacter species ygaZH genes, Shigella species ygaZH genes, Raoultella species ygaZH genes, Enterobacter species ygaZH genes, Yersinia species ygaZH genes, or Photorhabdus species ygaZH genes are overexpressed compared to the expression of said genes in the corresponding unmodified microorganism, and wherein the enhanced expression and the overexpression are achieved by:
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