Carbon fixation cycle and use thereof

An in vitro carbon dioxide (CO2) fixation cycle, consisting of an α-subunit and a β-subunit of a succinyl CoA synthetase having SEQ ID NOs:

• 19 and 20, isocitrate lyase having SEQ ID NO:
• 21, α-subunit and β-subunit of a 2-oxoglutarate synthase having SEQ ID NOs:
• 22 and 23, and isocitrate dehydrogenase having SEQ ID NO:
• 24,
• wherein:
• said α-subunit and β-subunit of said succinyl-CoA synthetase converts succinate into succinyl-CoA, said α-subunit and β-subunit of said 2-oxoglutarate synthase converts succinyl-CoA into 2-oxoglutarate, an isocitrate dehydrogenase converts 2-oxoglutarate into isocitrate, and said isocitrate lyase converts isocitrate into succinate and glyoxylate;
• a concentration of each of said succinate and said succinyl-CoA is maintained at ratio of 2:1 to 100:1,
• a concentration of each of said succinyl-CoA and said 2-oxoglutarate is maintained at ratio of 100:1 to 10,000:1;
• a concentration of each of sand 2-oxoglutarate and said isocitrate is maintained at ratio of 2:1 to 100:1;
• a concentration of each of said isocitrate and said glyoxylate is maintained at ratio of 1:10 to 1:1,000;
• and
• a fixation of carbon dioxide is carried out by carboxylation activity of one or more enzymes selected from the group consisting of said 2-oxoglutarate synthase and said isocitrate dehydrogenase.