US 10,190,125 B2Granted

Haploid induction compositions and methods for use therefor

AssigneeSyngenta Participations AG

InventorsSatya Chintamanani, Timothy Kelliher, Brent Delzer, Michael L. Nuccio, Robert Arthur Dietrich, Suresh Babu Kadaru, Todd Lee Warner, William Paul Bullock

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Abstract

Provided are isolated cDNAs comprising a nucleotide sequence having at least 90% identity to SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53. Also provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit HI; methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid-inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.

Claims2

Dependent claims

A method of creating a new haploid inducer maize plant with a silenced patatin-like phospholipase 2A, comprising transcribing a polynucleotide sequence that silences the patatin-like phospholipase 2A in maize, wherein said polynucleotide sequence comprises a first sequence selected from the group consisting of:

a) a polynucleotide sequence comprising the nucleic acid sequence set forth in SEQ ID NO:
33 or the complement thereof;
b) a functional fragment comprising at least 22 contiguous bases of SEQ ID NO:
33 or the complement thereof;
and
c) a polynucleotide sequence having at least 95% sequence identity as determined using the BLASTN alignment tool to the nucleic acid sequence set forth in SEQ ID NO:
33 or the complement thereof,
and a second sequence that is the complement of the first sequence, wherein the polynucleotide sequence expresses a double-stranded ribonucleotide sequence which silences the patatin-like phospholipase 2A when contacted with a maize plant and thus creates a new haploid inducer maize plant.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application: 15/586,649Filed 4 May 2017 · division
Priority: 15 Mar 2013
Published: US 2017/0067067 A124 Aug 2017
Granted: 29 Jan 2019
Term ends: Not estimatedThe source records don’t support an estimate.

Classification18 codes

C12N 15/8216
For plant cells , e.g. plant artificial chromosomes: methods for controlling, regulating or enhancing expression of transgenes in plant cells
A01H 1/045
Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection: using molecular markers
A01H 1/08
Processes for producing mutations, e.g. treatment with chemicals or with radiation: methods for producing changes in chromosome number
C12N 9/18
Acting on ester bonds: carboxylic ester hydrolases

Family

Application61/852,428Us provisional application · filed 15 Mar 2013
Application14/212,504Parent of this division · filed 14 Mar 2014
US 2017/0240912 A1Pre-grant publication · published 24 Aug 2017
US 9,677,082 B2Parent of this division
US 2017/0067067 A1Published application
US 10,190,125 B2This patent

From the US filing history. Foreign family members aren’t listed.