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US 10,188,750 B1Granted
Self-replicating cell selective gene delivery compositions, methods, and uses thereof
AssigneeUniversity of South Florida
InventorHana Totary-Jain
Life of this patent
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Abstract
Described herein are cell-selective mRNA constructs that can contain a RNA of interest and one or more miRNA targets. The cell-selective mRNA constructs described herein can be used to express an RNA of interest to a cell in a cell-selective manner.
Claims17
A polyribonucleotide comprising:
- a RNA molecule of interest (ROI), wherein the ROI is an RNA capable of being translated into p27;
- a microRNA (miRNA) target sequence, wherein the miRNA target sequence is a target for miR-126, and wherein the miRNA target sequence is operatively linked to the ROI;
- and
- a RNA molecule capable of being translated into a viral RNA replicase, wherein the RNA molecule capable of being translated into a viral RNA replicase is operatively linked to the ROI, the miRNA target sequence, or both the ROI and miRNA target sequence.
1 more element
A pharmaceutical formulation comprising:
- a polyribonucleotide comprising:
- a RNA molecule of interest (ROI), wherein the ROI is an RNA capable of being translated into p27;
- a microRNA (miRNA) target sequence, wherein the miRNA target sequence is a target for miR-126, and wherein the miRNA target sequence is operatively linked to the ROI;
- and
- a RNA molecule capable of being translated into a viral RNA replicase, wherein the RNA molecule capable of being translated into a viral RNA replicase is operatively linked to the ROI, the miRNA target sequence, or both the ROI and miRNA target sequence;
- and
- a pharmaceutically acceptable carrier.
4 more elements
A method comprising:
- administering a polyribonucleotide to subject, wherein the polyribonucleotide comprises
- a RNA molecule of interest (ROI), wherein the ROI is an RNA capable of being translated into p27;
- a microRNA (miRNA) target sequence, wherein the miRNA target sequence is a target for miR-126, and wherein the miRNA target sequence is operatively linked to the ROI;
- and
- a RNA molecule capable of being translated into a viral RNA replicase, wherein the RNA molecule capable of being translated into a viral RNA replicase is operatively linked to the ROI, the miRNA target sequence, or both the ROI and miRNA target sequence.
2 more elements
Dependencies are inferred from the claim text.