Microorganisms for producing glycogen and methods of using same
AssigneeWisconsin Alumni Research Foundation
InventorsBrian F. Pfleger, Andrew L. Markley
Life of this patent
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Abstract
Recombinant microorganisms configured for increased glycogen production. The recombinant microorganisms comprise a recombinant nucleic acid configured to express or overexpress a glucose-1-phosphate adenylyltransferase. The recombinant microorganisms produce an increased amount of glycogen compared to a corresponding microorganism not comprising the recombinant nucleic acid.
Claims20
A recombinant microorganism modified with respect to a native microorganism, the recombinant microorganism comprising a recombinant nucleic acid configured to express a glucose-1-phosphate adenylyltransferase, wherein the recombinant microorganism:
- is a cyanobacterium;
- exhibits enhanced glucose-1-phosphate adenylyltransferase activity compared to the native microorganism;
- exhibits a native glycogen synthase expression level;
- exhibits native glycogen synthase activity;
- and
- produces an increased amount of glycogen compared to the native microorganism while having a growth rate of at least a growth rate of the native microorganism when grown photoautotrophically in the presence of light and 10% CO2.
Dependencies are inferred from the claim text.