Mutated cellobiose dehydrogenase with increased substrate specificity
AssigneeDirectsens GmbH
InventorsRoland Ludwig, Christoph Sygmund, Wolfgang Harreither, Roman Kittl, Alfons Felice
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Abstract
The present invention relates to a modified cellobiose dehydrogenase (CDH) or its functional flavodehydrogenase domain having glucose oxidation activity and a reduced maltose oxidation activity as compared to the unmodified CDH or its functional flavodehydrogenase domain, nucleic acids encoding said enzyme or domain, electrodes with said enzyme or domain and methods of producing and using the same.
Claims13
A polypeptide comprising a modified flavodehydrogenase domain, which comprises a modification of at least one amino acid corresponding to any one of amino acids C312, N313, W316, R622 and/or N721 of SEQ ID NO:
- 1, or any combination thereof;
- wherein the flavodehydrogenase domain has an amino acid sequence of at least 80% sequence identity to a sequence selected from amino acids 251-828 of SEQ ID NO:
- 1, amino acids 263-839 of SEQ ID NO:
- 3, amino acids 253-831 of SEQ ID NO:
- 5, amino acids 249-787 of SEQ ID NO:
- 7, amino acids 251-829 of SEQ ID NO:
- 9, or amino acids 253-829 of SEQ ID NO:
- 11.
Dependencies are inferred from the claim text.