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US 10,184,106 B2Granted
Methods for viral inactivation and other adventitious agents
AssigneeGenentech, Inc.
InventorsMasaru Ken Shiratori, Robert David Kiss, Hardayal Prashad, Raquel Iverson, Justin Bourret, Michael Kim, Salim Charaniya
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Abstract
The invention provides for methods of viral inactivation using high temperature short time (HTST) treatment and adjustment of various parameters such that generation of precipitate and depositions of precipitate are reduced and/or minimized.
Claims25
A method for inactivating virus or adventitious agents in a mammalian cell culture media while the media maintains suitability for mammalian cell culture, said method comprising
- a) adjusting the pH of the cell culture media, wherein the cell culture media has a pH 5.0 to pH 6.9 prior to HTST treatment,
- b) subjecting the cell culture media to HTST treatment, and
- c) raising the pH of the cell culture media to between 6.9 and 7.2 after HTST treatment.
Dependencies are inferred from the claim text.