Cationic oil-in-water emulsions
AssigneeGlaxosmithkline Biologicals SA
InventorsLuis Brito, Michelle Chan, Andrew Geall, Derek O'Hagan, Manmohan Singh
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Abstract
This invention generally relates to cationic oil-in-water emulsions that contain high concentrations of cationic lipids and have a defined oil:lipid ratio. The cationic lipid can interact with the negatively charged molecule thereby anchoring the molecule to the emulsion particles. The cationic emulsions described herein are useful for delivering negatively charged molecules, such as nucleic acid molecules to cells, and for formulating nucleic acid-based vaccines.
Claims24
A composition comprising an RNA molecule and a stable oil-in-water emulsion comprising particles that are dispersed in an aqueous continuous phase, wherein the average diameter of said particles is from about 80 nm to about 150 nm;
- wherein the average diameter does not change by more than 10% when the emulsion is stored at 4° C. for one month;
- wherein the emulsion comprises an oil and a cationic lipid, wherein the head group of the cationic lipid comprises a quaternary amine;
- and wherein:
- (i) the ratio of oil:lipid (mole:mole) in the oil-in-water emulsion is at least about 8:1 (mole:mole);
- (ii) the concentration of cationic lipid in said composition is at least about 1.25 mM;
- (iii) the cationic lipid is not DC-Cholesterol, and
- (iv) the RNA molecule is complexed with a particle of the oil-in-water emulsion.
Dependencies are inferred from the claim text.