Method of using a transgenic chicken to produce exogenous proteins in its eggs

A method of using a transgenic chicken to produce an exogenous protein comprising:

• providing a transgenic chicken whose genome comprises a self-inactivating avian leukosis viral (ALV) vector encoding an exogenous protein operably linked to a promoter;
• obtaining a hard shell egg containing the exogenous protein from the transgenic chicken,
• wherein the promoter comprises a nucleic acid sequence that is at least 90% identical to a nucleic acid molecule comprising from 5′ to 3′:
• i) an ovalbumin Dnase hypersensitive site (DNS) III consisting of nucleotides 3253-3559 of SEQ ID NO:
• 22;
• ii) an ovalbumin DNS II consisting of nucleotides 5629-6009 of SEQ ID NO:
• 22;
• iii) an ovalbumin DNS I consisting of nucleotides 6359-6659 of SEQ ID NO:
• 22;
• iv) a 5′ UTR-5′ portion from Exon L consisting of nucleotides 6659-6705 of SEQ ID NO:
• 22;
• v) intron A consisting of nucleotides 6706-8294 of SEQ ID NO:
• 22;
• vi) a 5′ UTR-3′ portion from Exon I consisting of nucleotides 8295-8311 of SEQ ID NO:
• 22;
• vii) a 3′ UTR.